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KMID : 1020220140140050775
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Journal of Korean Society of Dental Hygiene
2014 Volume.14 No. 5 p.775 ~ p.781
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The protective effect of coenzyme Q10 on cytotoxicity of regin monomer of odontoblast caused by TEGDMA
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Lee Ah-Reum
Park So-Yeong
Lee Kyung-Hee
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Abstract
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Objectives : The purpose of the study is to investigate the protective effect of coenzyme Q10 on cytotoxicity effect of dental monomers in odontoblast(MDPC-23).
Methods : MDPC-23 was incubated with the(co)monomers triethylene glycol dimethacrylate (TEGDMA) with and without addition of coenzyme Q10. The cell proliferation and survival was determined using WST-1 assay. The level of reactive oxygen species(ROS) was measured by immunofluorescent staining for DCF-DA.
Results : TEGDMA treatment decreased the cell proliferation by dose dependently(0.1, 1, 2.5, 5, 10 mM) on the growth of MDPC-23 cells. Coenzyme Q10 showed cell proliferation from 5 to 500 μM by WST-1 assay. Pre-treatment coenzyme Q10 showed the antioxidant effect on proliferation and viability of MDPC-23 after 48h(p<0.05). The positive cells were observed in non-coenyme Q10 treatment group(group 2) in comparison with coenyme Q10 pre-treatment group(group 1) by DCF-DA. The fluorescence positive cells showed 14.715(group 1) and 19.788(group 2) using image J system.
Conclusions : TEGDMA induced cytotoxicity. The MDPC-23 cell death was associated with the increasing ROS. Coenyme Q10 showed the antioxidant effects by decreasing ROS. This effects may contribute to the treatment of periodontal disease induced by TEGDMA after operation.
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KEYWORD
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antioxidant, cell death, coenzyme Q10, odontoblast, triethylene glycol dimethacrylate
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